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New Perspectives in Science Education 12th Edition 2023

Course-based undergraduate research experience for biochemistry majors in physical chemistry

Florentina Arispe; Andrés Ansin; Tatiana Montagni; María Fernanda Cerdá; Santiago Botasini; Eduardo Méndez

Abstract

A course-based undergraduate experience (CURE) was designed for for biochemistry majors in physical chemistry, based on 15 years of prior experience [1-3]. The course includes a theoretical background in classical thermodynamics and kinetics, problem-solving, and laboratory experiments. In the laboratory part, students follow a fairly comprehensive study of different proteins, from analytical evaluation by UV/VIS spectrometry, followed by transport measurements, differential scanning calorimetry, denaturation induced by chemical products, denaturation induced by heat, and kinetics of denaturation. These laboratory proposals allow the student to gather all the information in a single study report, and different proteins can be included, giving rise to the need for the selection of specific methods by the students [4]. Our entire presentation will be based on the phycocyanin protein form Spirulina platensis.

Keywords

CURE, kinetics, proteins, thermodynamics

References

[1] Pereyra, M., et al., “Picturing the thermodynamic universe with Frida Kahlo”, J. Phys. Conf. Ser., 2021, 1938, 045603

[2] Méndez, E., Cerdá, M.F., “Discovering reliable sources of biochemical thermodynamics data to aid student’s understanding”, J. Chem. Educ. 2016, 93, 555-559

[3] Méndez, E., “Biochemical Thermodynamics”, in Advances in Biology, 2022, Vol. 1, 151-183, ISBN : 979-8-88697-201-6

[4] Cooper, K.M., et al., “The impact of broadly relevant novel discoveries on student project ownership in a traditional lab course turned CURE”, CBE Life Sciences Education, 2022, ar57

 


Publication date: 2023/03/17
ISBN: 979-12-80225-55-9
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